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KMID : 0043320120350122153
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Archives of Pharmacal Research
2012 Volume.35 No. 12 p.2153 ~ p.2162
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High-performance liquid chromatographic analysis for quantitation of marker compounds of Artemisia capillaris thunb.
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Park Kyung-Min
Li Ying
Kim Bo-Ra
Zhang HaiYan
Hwangbo Kyong
Piao Dong-Gen
Chi Mei Juan
Woo Mi-Hee
Choi Jae-Sue
Lee Je-Hyun
Moon Dong-Cheul
Chang Hyeun-Wook
Kim Jae-Ryong
Son Jong-Keun
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Abstract
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Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among ‘Injinho’ and ‘Myeon-injin’ and ‘Haninjin’ ? A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as ‘Injinho’ in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognition analysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
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KEYWORD
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Artemisia capillaris Thunb., Chlorogenic acid, 3, 5-Di-O-caffeoylquinic acid, 3, 4- Di-O-caffeoylquinic acid, Scoparone, HPLC
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